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KMID : 1025520040460010001
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Journal of Animal Science and Technology
2004 Volume.46 No. 1 p.1 ~ p.6
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Detection of Novel Genetic Variations of the MC1R *3 Allele in Pig(Sus scrofa)
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조인철/Cho, I. C.
정용환/정진관/성필남/오운용/고문석/김병우/Jung, Y. H./Jung, J. K./Seung, P. N./Oh, W. Y./Ko, M. S./Kim, B. W.
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Abstract
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This study was conducted to investigate novel genetic variations of MCIR/R*3 allele. In general, white spotting or white belt on a black backgroud in pigs is determined by the E^(p) allele at the MICR/Extention locus. E^(p) shares a frameshift mutation with the E^(p) allele for dominant black color. An oligonucleotide primer set was designed to amplify complete coding sequence of the porcine MICR gene. The MICR coding sequences obtained from five breeds those were Landrace(white), Yorkshire(white), Hampshire(belt), Berkshire(spot) and Jeju native black pigs(black), were used for this study. A multiple sequence alignment of the MICR coding region using Clustal W was performed. The total length of the MCIR coding sequence ranged from 963 to 966 base pairs(bp) among the selected breeds. The sequence analysis of the complete coding region of MICR was revealed that Hampshire and Jeju native black pig have 3 cytosines deletion and Birkshire has 2 cytosines deletion at codon 23(nt68) in Extention loci. Besides the finding, there were three different missense mutations and a frameshift mutation in the MCIR coding region.
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